pathogens & biofilm (7)

7 Jun 2017

First Report of Pythium aphanidermatum Causing Root Rot and Decline of Poinsettia in Maryland

Del Castillo Múnera, J. (University of Maryland) and C.L. Swett (University of California, Davis)

The publication is one of the outcomes from the statewide monitoring of water-borne oomycetes to provide critical control point assessment to nursery producers. Pythium aphanidermatum was isolated from poinsettia exhibiting root rot symptoms.  Healthy poinsettia were inoculated in a greenhouse experiment with isolates recovered from the root rot resulted in wilting and root rot symptoms.  P. aphanidermatum was recovered, confirming that it is a poinsettia pathogen in Maryland.  This new information enables regional diagnosticians and crop advisors to provide more accurate information to producers, and facilitates research efforts to improve root disease management.

See the link bellow

http://apsjournals.apsnet.org/doi/10.1094/PDIS-11-16-1671-PDN

26 May 2017

Next Generation Sequencing of Oomycete Communities in Nursery Irrigation Water

Eberhart, J., Funahashi, F., Foster, Z.S.L., Parke, J. (Oregon State University)

Our current research is focused on helping plant nurseries monitor oomycete pathogens in their irrigation water to determine the need for water treatment, evaluate effectiveness of treatment options, and enable selection of cost-effective ways to disinfest water. Once protocols have been fully developed and validated, Illumina sequencing has the potential to be a sensitive method to detect, identify, and estimate the relative abundance of oomycete communities from water samples. We will use this knowledge to help nursery managers make informed decisions about effective water disinfestation strategies, reducing the risk of establishment of plant pathogens.

Sudden Oak Death 6th Science Symposium June 2016 (2554 KB)

6 Feb 2017

Elimination of Tobacco Mosaic Virus From Irrigation Runoff Using Slow Sand Filtration

Oki, L.R., Lee, E., Pitton, B., Nackley, L.,(University of California (UC) Davis), Bodaghi, S., Mathews, D.M. (UC Riverside), Haver, D. (UC South Coast Research and Extension Center)

Slow sand filters have been shown to remove pythiacious organisms from captured runoff water.  In this study, Tobacco Mosaic Virus (TMV) was regularly added to irrigation runoff water.  The virus passed through the sand filters for approximately 5 weeks, but gradually reduced to undetectable virus titer using ELISA during week 6 to 9.  This is the first report that showed slow sand filters removed TMV from runoff water.

See the article in the link below

http://dx.doi.org/10.1016/j.scienta.2017.01.036

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Description of research activities

A national team of scientists is working to encourage use of alternative water resources by the nation’s billion-dollar nursery and floriculture industry has been awarded funds for the first year of an $8.7 million, five year US Department of Agriculture – National Institute of Food and Agriculture –Specialty Crop Research Initiative competitive grant.

The team will develop and apply systems-based solutions to assist grower decision making by providing science-based information to increase use of recycled water.  This award from the NIFA’s Specialty Crop Research Initiative is managed by Project Director Sarah White of Clemson University.  She leads a group of 21 scientists from nine U.S. institutions.

Entitled “Clean WateR3 - Reduce, Remediate, Recycle – Enhancing Alternative Water Resources Availability and Use to Increase Profitability in Specialty Crops”, the Clean WateR3 team will assist the grower decision-making process by providing science-based information on nutrient, pathogen, and pesticide fate in recycled water both before and after treatment, average cost and return-on investment of technologies examined, and model-derived, site specific recommendations for water management.  The trans-disciplinary Clean WateR3 team will develop these systems-based solutions by integrating sociological, economic, modeling, and biological data into a user-friendly decision-support system intended to inform and direct our stakeholders’ water management decision-making process.

The Clean WateR3 grant team is working with a stakeholder group of greenhouse and nursery growers throughout the United States.

For example, at the University of Florida graduate student George Grant is collecting data on removal of paclobutrazol, a highly persistent plant growth regulator chemical, from recirculated water using granular activated carbon (GAC) filters. This is being done in both research greenhouses and in a commercial site. The GAC filters can remove more than 90% of chemical residues, and are proving to be a cost-effective treatment method.

 

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